Publikationen

Fachartikel

Developmental validation of the monSTR identity panel, a forensic STR multiplex assay for massively parallel sequencing
Silvery J, Ganschow S, Wiegand P, Tiemann C
Forensic Science International: Genetics 46 (2020), 102236

Development of a multiplex forensic identity panel for massively parallel sequencing and its systematic optimization using design of experiments
Ganschow S, Silvery J, Tiemann C
Forensic Science International: Genetics 39, 32-43 (2019)
Online-Artikel & PDF

toaSTR: A web application for forensic STR genotyping by massively parallel sequencing
Ganschow S, Silvery J, Kalinowski J, Tiemann C
Forensic Science International: Genetics 37, 21-28 (2018)

toaSTR: A web-based forensic tool for the analysis of short tandem repeats in massively parallel sequencing data
Ganschow S, Wiegand P, Tiemann C
Forensic Science International: Genetics Supplement Series, 2017

Establishment of a voluntary electronic Chlamydia trachomatis laboratory surveillance system in Germany, 2008 to 2014
Dudareva-Vizule S, Haar K,Sailer A, Jansen K, Hamouda O, Wisplinghoff H, Tiemann C, Pape E, Bremer V, Chlamydia trachomatis laboratory sentinel team
Eurosurveillance 22 (2017)

Prevalence of Chlamydia trachomatis infection in women, heterosexual men and MSM visiting HIV counselling institutions in North Rhine-Westphalia, Germany – should Chlamydia testing be scaled up?
Lallemand A, Bremer V, Jansen K, Nielsen S, Münstermann D, Lucht A, Tiemann C
BMC Infectious Diseases 16 (2016)

Local Health Study: Aufsuchende medizinische Angebote für Sexarbeiterinnen in Bochum
Klingenberg RE, Mannherz S, Brockmeyer NH, Wach J, Winter R, Tiemann C, Lucht A, Schaffer S, Eickel M, Skaletz-Rorowski A
Der Hausarzt 67 (2016)

Go Local Health Study: Aufsuchende medizinische Angebote bei Sexarbeiterinnen in Bochum
Scheitz RE, Mannherz S, Wach J, Winter R, Eickel M, Tiemann C, Brockmeyer NH
JDDG (2015)

Draft genome sequence of Bacillus anthracis UR-1 isolated from a
German heroin user
Rückert C, Licht K, Kalinowski J, Santo CE, Antwerpen M, Hanczaruk M, Reischl U, Holzmann T, Gessner A, Tiemann C, Grass G
Journal of Bacteriology (2012)

Draft genome sequence of Bacillus anthracis BF-1 isolated from Bavarian cattle
Antwerpen M, Proença D, Rückert C, Licht K, Kalinowski J, Hanczaruk M, Tiemann C, Grass G
Journal of Bacteriology (2012)

H1N1, der Erreger des Jahres 2009 – Pandemie ohne Panik
Tiemann C
Trillium Report (2009)

A Cryptographic Biometric Authentication System based on Genetic Fingerprints
Korte U, Krawczak M, Merkle J, Plaga R, Niesing M, Tiemann C, Vinck H, Martini U
Sicherheit, 263-276 (2008)

Frequency of very low HCV viremia detected by a highly sensitive HCV-RNA assay
Fytili P, Tiemann C, Wang C, Schulz S, Schaffer S, Manns MP, Wedemeyer H
J Clin Virol. 39(4), 308-11 (2007)

Multicenter evaluation of the new Abbott RealTime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA
Schutten M, Peters D, Back NK, Beld M, Beuselinck K, Foulongne V, Geretti AM, Pandiani L, Tiemann C, Niesters HG
J Clin Microbiol. 45(6), 1712-7 (2007)

Detection of Treponema pallidum in the vitreous by PCR
Müller M, Ewert I, Hansmann F, Tiemann C, Hagedorn HJ, Solbach W, Roider J, Nölle B, Laqua H, Hoerauf H
Br J Ophthalmol. 91(5), 592-5 (2007)

Ultra-rapid detection of Chlamydia trachomatis by real-time PCR in the LightCycler using SYBR green technology or 5′-nuclease probes
Eickhoff M, Laue T, Ruckes T, Cramer SO, Krupp G, Tiemann C
Clin Lab. 49(5-6), 217-25 (2003)

Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology
Tiemann C, Vogel A, Dufaux B, Zimmer M, Krone JR, Hagedorn HJ
Clin Lab. 47(3-4), 131-4 (2001)

Effect of cyclosporine A on the release of tissue factor pathway inhibitor from endothelial cells in heart transplant patients and cell culture
Tiemann C, Prohaska W, Körfer R, Körner M, Brinkmann T, Kleesiek K
Eur J Clin Chem Clin Biochem. 35(9), 661-7 (1997)

Detection of the three Kunitz-type single domains of membrane-bound tissue factor pathway inhibitor (TFPI) by flow cytometry
Tiemann C, Brinkmann T, Kleesiek K
Eur J Clin Chem Clin Biochem. 35(11), 855-60 (1997)

Genomic organization and sequence of the rat major histocompatibility complex class Ia gene RT1.Au
Walter L, Tiemann C, Heine L, Günther E
Immunogenetics. 41(5), 332 (1995)

Results from an IFCC global survey on laboratory practices for the analysis of circulating tumor DNA
Mark W. Linder, Jim F. Huggett, Katarina Baluchova, Ettore D. Capoluongo, Deborah A. Payne, Aldo Vacaflores Salinas, Verena Haselmann, Tester Ashavaid, Shiyang Pan, Parviz Ahmad-Nejad, IFCC Committee for Molecular Diagnostics C-MD

Clinica Chimica Acta, Volume 547 (2023), 117398

Molecular diagnostics of SARS-CoV-2: Findings of an international survey
Aldo Vacaflores Salinas, Tester Ashavaid, Deborah A. Payne, Mark W. Linder, Katarina Baluchova, Shiyang Pan, Jim Huggett, Parviz Ahmad-Nejad, the IFCC Committee for Molecular Diagnostics C-MD

Clinica Chimica Acta, Volume 531 (2022), 237-242

Recommendations for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samples
Kubik, AC. Marques, X. Xing, J. Silvery, C. Bertelli, F. De Maio, S. Pournaras, T. Burr, Y. Duffourd, H. Siemens, C. Alloui, L. Song, Y. Wenger, A. Saitta, M. Macheret, EW. Smith, P. Menu, M. Brayer, LM. Steinmetz, A. Si-Mohammed, J. Chuisseu, R. Stevens, P. Constantoulakis, M. Sali, G. Greub, C. Tiemann, V. Pelechano, A. Willig, Z. Xu.

Clinical Microbiology and Infection (2021) Jul;27(7): 1036.e1-1036.e8.

Buchtitel

Die Detektion von PCR Produkten
Tiemann C
in Leitfaden Molekulare Diagnostik (Ed. Thiemann, Cullen, Klein) Wiley-VCH (2006)

Die Detektion von PCR Produkten
Tiemann C
in Leitfaden Molekulare Diagnostik, 2. Auflage (Ed. Thiemann, Cullen, Klein) Wiley-VCH (2015)

Posterpräsentationen

Development and validation of the monSTR forensic identity panel – a multiplex STR Kit for MPS
Silvery J., Ganschow S., Siemens H., Wiegand P., Tieman C.
28th Congress of the International Society for Forensic Genetics, Prag (2019)

Application of a multiplex PCR assay for detection of sexually transmitted infections
Kulis-Horn RK, Tiemann C
10th EMMD, Noordwijk (2017)

Automatisierungslösungen im PCR-Labor
Tiemann C
Seegene Symposium, Kassel (2017)

STI Diagnostik I/II
Kulis-Horn RK
Seegene Symposium, Kassel (2017)

Multiplex-PCRs (Gastro, Respi, STI) in der klinischen Anwendung
Tiemann C
6. REMMDI, Regensburg (2017)

Implant-associated Infection Diagnosis using Sonication and Molecular Methods
Ganschow S, Kalinowski J, Tiemann C
11th CeBiTec Symposium, Bielefeld (2016)

Implant-associated Infection Diagnosis Using Sonication and High-Throughput 16S rRNA Gene Sequencing: A Standardisation and Validation Approach
Ganschow S, Kalinowski J, Tiemann C
26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Amsterdam (2016)

Prevalence of Mycoplasma genitalium in patients visiting HIV counselling institutions in North-Rhine-Westphalia, Germany (STI-HIT study)
Lallemand A, Bremer V, Jansen K, Nielsen S, Münstermann D, Lucht A, Tiemann C
ISSTDR (2015)

High prevalence of genital infections with Mycoplasma genitalium in female sex workers reached at their working place in Germany: the STI-Outreach study
Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S, Münstermann D, Lucht A, Tiemann C
ISSTDR (2015)

High prevalence of genital infections with Chlamydia, gonorrhoea and trichomonas in female sex workers reached at their working place in Germany: the STI-Outreach study
Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S, Münstermann D, Lucht A, Tiemann C
ECCMID (2015)

Prävalenz genitaler Infektionen mit Chlamydia trachomatis (CT), Neisseria gonorrhoea (NG) und Trichomonas vaginalis (TV) in Klienten der HIV-Testberatungsstellen in Nordrhein-Westfalen: die STI-HIT Studie
Bremer V, Jansen K, Steffen G, Sarma N, Nielsen S, Lucht A, Münstermann D, Tiemann C
DGSTI (2014)

Hohe Prävalenz genitaler Infektionen mit Chlamydien, Gonorrhö und Trichomonaden bei am Arbeitsplatz erreichten Sexarbeiterinnen in Deutschland: die STI-Outreach-Studie
Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S, Münstermann D, Lucht A, Tiemann C,  DGSTI (2014)

Prevalence of genital infections with Chlamydia trachomatis, Neisseria gonorrhea and Trichomonas vaginalis in hard-to-reach sex workers in Germany: The STI-Outreach Study
Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S, Münstermann D, Lucht A, Tiemann C
DÖÄK (2013)

Prevalence of  genital infections with Chlamydia trachomatis, Neisseria gonorrhoea and Trichomonas vaginalis in clients of public HIV counseling institutions in North Rhine-Westphalia, Germany: the STI-HIT study
Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S, Münstermann D, Lucht A, Tiemann C
DÖÄK (2013)

Comparison of PCR and electrospray-ionization mass spectrometry (ESI-MS) with
species-specific diagnostic algorithms during a biodefense external quality
assurance exercise
Thoma BR, Knoop D, Hartmann A, Tiemann C, Zöller L, Hinz C, Jacob D, Grunow R, Schmoldt S
DGHM Jahrestagung (2012)

Evaluation of analytical sensitivity and specificity of the biothreat assay for clinical Bacillus anthracis diagnostics by the PLEX-ID™ System
Hanczaruk M, Thoma BR, Antwerpen M, Schmoldt S, Tiemann C, Knoop D, Hartmann A, Zöller L, Grass G
VAAM Jahrestagung (2012)

PlexID System: evaluation of analytical sensitivity and specificity of the biothreat assay for clinical Bacillus anthracis diagnostics
Thoma BR, Hanczaruk M, Antwerpen MH, Schmoldt S, Knoop D, Hartmann A, Tiemann C, Grass G
Medical Biodefence Conference (2012)

Identification of orthopoxviruses by PCR/electrospray-ionization mass spectrometry
Meyer H, Dobrzykowski L, Knoop D, Hartmann A, Thoma BR, Tiemann C, Schmold S
Medical Biodefence Conference (2012)

Rapid identification of infectious pathogens with PCR/electrospray-ionization TOF mass spectrometry
Tiemann C, Knoop D, Hartmann A, Busse C
European Meeting on Molecular Diagnostics (2011)

Improvement of a RNA isolation method for carotid endarterectomy samples embedded in cryo-medium
Knoop D, Lanckohr C, Theilmeier G, Tiemann C
European Meeting on Molecular Diagnostics (2009)

Evaluation of the KingFisher Flex© for high throughput extraction of DNA and RNA from clinical samples
Hartmann A, Knoop D, Zinn T, Tiemann C
European Meeting on Molecular Diagnostics (2009)

Evaluation of testing cadaveric serum for HIV, HCV and HBV viraemia
Tiemann C, Knoop D
European Meeting on Molecular Diagnostics (2007)

Performance Evaluation of a New Instrument for Genomic orViral DNA or RNA Extraction for HBV Viral Load Testing
Tiemann C, Knoop D, Dunn C, Schaffer S
European Meeting on Molecular Diagnostics (2007)

Rapid Detection of Norovirus Genogroups By Automated RNA-Extraction and Real-time RT-PCR
Tiemann C, Vogel A, Dufaux B, Zimmer M, Münstermann D, Krone JR, Hagedorn HJ
European Meeting on Molecular Diagnostics (2005)

Rapid and sensitive detection of PCR products in clinical diagnostics using the electrophoresis system Caliper 1000
Tiemann C, Vogel A, Dufaux B, Zimmer M, Münstermann D, Krone JR, Hagedorn HJ
European Meeting on Molecular Diagnostics (2003)

Rapid Detection of Chlamydia trachomatis by Real-time PCR Using SYBR-Green I
Tiemann C, Vogel A, Dufaux B, Zimmer M, Münstermann D, Krone JR, Hagedorn HJ
European Meeting on Molecular Diagnostics (2001)

Vorträge

SWGDAM Validationsstudie des monSTR Panels – ein Multiplex Kit für die hochauflösende STR-Typisierung mittels MPS
Silvery J
15. Jahrestagung der Deutschen Gesellschaft für Abstammungsbegutachtung (DGAB), Köln (2019)

Design of Experiments: Wie aus monSTR ein High-Performance Identity Panel wurde
Ganschow S
39. Spurenworkshop, Jena (2019)

Massively Parallel Sequencing für die hochauflösende STR-Typisierung – Validation des 21-plex Kits monSTR
Silvery J
14. Jahrestagung der Deutschen Gesellschaft für Abstammungsbegutachtung (DGAB), Freiburg (2018)

toaSTR: a user-friendly tool for STR allele calling in MPS data
Ganschow S
ENFSI DNA Working Group Meeting, Rom (2018)

toaSTR: die Web-App für eine einfache und effiziente STR-Genotypisierung mit MPS
Ganschow S
38. Spurenworkshop, Basel (2018)

Der Sequenz auf der Spur: MPS-Konkordanzstudie zum GEDNAP-Ringversuch
Silvery J
38. Spurenworkshop, Basel (2018)

toastr.de – the Swiss knife for next-generation STR genotyping
Ganschow S
18th European Forensic DNA Working Group Meeting, Innsbruck (2017)

NGS in forensics – from complex to simple
Tiemann C

18th European Forensic DNA Working Group Meeting, Innsbruck (2017)

toaSTR: a web-based forensic tool for the analysis of short tandem repeats in massively parallel sequencing data
Ganschow S
27th Congress of the ISFG, Seoul (2017)

Praktische Erfahrungen mit dem PantherFUSION System
Tiemann C
Hologic Anwender-Treffen (2017)

Application of multiplex real-time PCR assays for STI patients
Tiemann C
ECCMID, Wien (2017)

Next-Generation Sequencing für die forensische Genetik: Entwicklung und Evaluation eines 17-plex PCR-Kits
Ganschow S

37. Spurenworkshop German DNA Profiling (GEDNAP), Gießen (2017)

Next Generation Sequencing für die forensische Genetik: Entwicklung und Evaluation eines High-Fidelity 10-plex STR-Assays
Ganschow S

12. Jahrestagung der Deutschen Gesellschaft für Abstammungsbegutachtung (DGAB), Bielefeld (2016)

Implant-associated Infection Diagnosis using Sonication and Molecular Methods
Ganschow S

11th CeBiTec Symposium, Bielefeld (2016)

Prävalenz genitaler Infektionen in verschiedenen Risiko-Populationen.
Tiemann C
Hologic Anwender-Treffen (2015)

Laborvergleich für Aptima Combo 2® External Quality Assessment for HOLOGIC Panther® and Tigris® System   Nucleic Acid Detection for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG)
Tiemann C
Hologic Anwender-Treffen (2015)

Genitale Infektionen mit Chlamydien, Gonorrhö und  Trichomonas bei „hard-to-reach“ Sexarbeiterinnen in Deutschland: Ergebnisse der STI-Outreach-Studie
Jansen K, Bremer V, Nielsen S, Steffen G, Sarma N, Tiemann C, Münstermann D, Lucht A, Schaffer S
NRW-LIGA-Meeting (2014)

Möglichkeiten und Grenzen automatisierter Nachweissysteme in der molekularen Diagnostik
Tiemann C
STI-Jubiläumskongress der DSTIG, Berlin (2012)

PlexID – zukünftige Möglichkeiten zur Erweiterung der Routinediagnostik beim Nachweis von Infektionserregern
Tiemann C
10. Wissenschaftliches Abbott Symposium (2012)

Identification of orthopoxviruses by PCR/electrospray-ionization mass spectrometry
Meyer H, Dobrzykowski L, Knoop D, Hartmann A, Thoma BR, Tiemann C, Schmold S,
Medical Biodefence Conference (2012)

Rapid identification of infectious pathogens with PCR/electrospray-ionization TOF mass spectrometry.
Tiemann C, Knoop D, Hartmann A, Busse C
European Meeting on Molecular Diagnostics (2011)

Implementierung der PCR/ESI-TOF MS Technologie im Routinelabor
Tiemann C
DGHM Tagung (2011)

Rapid identification of mixed- and single bacterial and fungal infections with PCR coupled to electrospray-ionization TOF mass spectrometry
Tiemann C, Knoop D, Hartmann A
DGHM Tagung 2011

Molekulare Identifizierung von Pathogenen mittels PCR / ESI-TOF Massenspektrometrie
Tiemann C
Regensburger Meeting für angewandte Molekulare Diagnostik (REMMDI) (2011)

Identifizierung und Charakterisierung von Bakterien und Viren mittels PlexID – Evaluierungsbericht.
Tiemann C
7. Abbott Anwender-Forum Molekulare Diagnostik (2011)

Massenspektometrische Untersuchung von STR-Systemen mittels ESI-TOF-MS/PCR – am Beispiel des aktuellen GEDNAP-Ringversuches
Tiemann C
31. Spurenworkshop der Deutschen Gesellschaft für Rechtsmedizin (2011)

RealTime High Risk HPV Diagnostik : erste Praxiserfahrung
Tiemann C
5. Abbott Anwender-Forum (2009)

Abbott Molecular Automation Advisory Board (Co-Chair)
Tiemann C
Amsterdam-Meeting (2010)

„Neue Grippe“ – H1N1
Tiemann C
Rotary Club Herford (2009)

Abbott RealTime High Risk HPV vs HC“ (Digene)
Tiemann C
Fortbildungstagung Klinische Zytologie, München (2009)

Chlamydien-Screening: erster Erfahrungsbericht
Tiemann C
4. Abbott Anwender-Forum (2008)

Gen-Profile und Identifizierungswahn – Möglichkeiten und moralische Grenzen
Tiemann C
FoeBuD (2008)